Lev Delaunay is applied after cells have been arrested during cell division by a solution of colchicine usually in metaphase or prometaphase when most condensed.In order for the Giemsa stain to adhere correctly, all chromosomal proteins must be digested and removed.
The study of karyotypes is important for cell biology and genetics, and the results may be used in evolutionary biology (karyosystematics) and medicine.
The diploid number of the Chinese muntjac, Muntiacus reevesi, was found to be 46, all telocentric.
When they looked at the karyotype of the closely related Indian muntjac, Muntiacus muntjak, they were astonished to find it had female = 6, male = 7 chromosomes. They kept quiet for two or three years because they thought something was wrong with their tissue culture ...
Although much is known about karyotypes at the descriptive level, and it is clear that changes in karyotype organization has had effects on the evolutionary course of many species, it is quite unclear what the general significance might be.
A spectacular example of variability between closely related species is the muntjac, which was investigated by Kurt Benirschke and his colleague Doris Wurster.